This molecular study of wastewater samples highlights the importance of validating taxonomy of NGS sequences with databases such as NCBI nr/nt, and recommends including the V3 region of 16S in future short amplicon NGS studies that aim to identify bacterial enteric pathogens in similar matrices. We claim this will improve taxonomic resolution of most, but not all, Enterobacteriaceae species.


Citation: T. L. Greay et al., Evaluation of 16S next-generation sequencing of hypervariable region 4 in wastewater samples: An unsuitable approach for bacterial enteric pathogen identification. Science of The Total Environment 670, 1111-1124 (2019).